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Fig. 4. Differences in the expression of VLA4/VCAM-1/RAC1/p-PYK2/p-VE-cad between control group and RHD group. (A,B) WB analysis of VLA4, VCAM-1, RAC1, p-PYK2 and p-VE-cad. The protein expression levels of VLA4, VCAM-1, RAC1, p-PYK2

Journal: Frontiers in bioscience (Landmark edition)

Article Title: Potential Involvement of M1 Macrophage and VLA4/VCAM-1 Pathway in the Valvular Damage Due to Rheumatic Heart Disease.

doi: 10.31083/j.fbl2906219

Figure Lengend Snippet: Fig. 4. Differences in the expression of VLA4/VCAM-1/RAC1/p-PYK2/p-VE-cad between control group and RHD group. (A,B) WB analysis of VLA4, VCAM-1, RAC1, p-PYK2 and p-VE-cad. The protein expression levels of VLA4, VCAM-1, RAC1, p-PYK2

Article Snippet: The primary antibodies used for immunohistochemistry were as follows: CD86 (1:80; cat. no. 13395-1-AP; ProteinTech Group, Inc., Wuhan, China), TNF-α (1:80; cat. no. 26405-1-AP; ProteinTech Group, Inc., Wuhan, China), VLA4 (1:80; cat. no. 19676-1-AP; ProteinTech Group, Inc., Wuhan, China), VCAM-1 (1:80; cat. no. DF6082; Affinity Biosciences, Jiangsu, China), p-PYK2 (1:80; cat. no. AF7383; Affinity Biosciences, Jiangsu, China), p-VE-cad (1:80; cat. no. AF3265; Affinity Biosciences, Jiangsu, China), interleukin (IL)-6 (1:65; cat. no. GB11117; Servicebio, Wuhan, China), and IL-17 (1:90; cat. no. GB11110-1; Servicebio, Wuhan, China).

Techniques: Expressing, Control

Fig. 5. Differences in the expression of VLA4/VCAM-1/RAC1/p-PYK2/p-VE-cad and endothelial permeability between M0+EC

Journal: Frontiers in bioscience (Landmark edition)

Article Title: Potential Involvement of M1 Macrophage and VLA4/VCAM-1 Pathway in the Valvular Damage Due to Rheumatic Heart Disease.

doi: 10.31083/j.fbl2906219

Figure Lengend Snippet: Fig. 5. Differences in the expression of VLA4/VCAM-1/RAC1/p-PYK2/p-VE-cad and endothelial permeability between M0+EC

Article Snippet: The primary antibodies used for immunohistochemistry were as follows: CD86 (1:80; cat. no. 13395-1-AP; ProteinTech Group, Inc., Wuhan, China), TNF-α (1:80; cat. no. 26405-1-AP; ProteinTech Group, Inc., Wuhan, China), VLA4 (1:80; cat. no. 19676-1-AP; ProteinTech Group, Inc., Wuhan, China), VCAM-1 (1:80; cat. no. DF6082; Affinity Biosciences, Jiangsu, China), p-PYK2 (1:80; cat. no. AF7383; Affinity Biosciences, Jiangsu, China), p-VE-cad (1:80; cat. no. AF3265; Affinity Biosciences, Jiangsu, China), interleukin (IL)-6 (1:65; cat. no. GB11117; Servicebio, Wuhan, China), and IL-17 (1:90; cat. no. GB11110-1; Servicebio, Wuhan, China).

Techniques: Expressing, Permeability